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Registros recuperados: 80
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Diagnostic quantitatif de Perkinsus atlanticus (Apicomplexa, Perkinsea) chez les palourdes Ruditapes decussatus et Ruditapes philippinarum ArchiMer
Almeida, Manuela.
Le protozoaire parasite Perkinsus atlanticus (Azevedo, 1989) est une des causes des chutes de production de palourdes Ruditapes decussatus et R. philippinarum cultivées au Portugal, en Espagne et en Italie. Des modèles d'infection de Perkinsus ont été réalisés, par reproduction expérimentale de la maladie, pour étudier le rôle de la densité des palourdes, de la température et de la salinité sur le déroulement de la Perkinsiose. L'objectif de ce travail a été de développer une technique de diagnostic quantitative pour optimiser l'évaluation de l'intensité d'infection et détecter de faibles infections des palourdes par P. atlanticus. Le principe de cette technique consiste à cultiver tous les tissus de la palourde en milieu thioglycolate, suivi d'une lyse à...
Tipo: Text Palavras-chave: Pathologie; Protozoaire; Parasite; Perkinsus atlanticus; Bivalvia; Ruditapes decussatus; Ruditapes philippinarum; Diagnostic.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00033/14416/11710.pdf
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One Perkinsus species may hide another: characterization of Perkinsus species present in clam production areas of France ArchiMer
Arzul, Isabelle; Chollet, Bruno; Michel, Justine; Robert, Maeva; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Miossec, Laurence.
Although clam populations in France are known to be infected with protozoans of the genus Perkinsus, no molecular characterization was previously performed on these parasites. Considering that several members of this genus have been associated with mortalities of molluscs worldwide, a study was undertaken in order to characterize these parasites in France. For that purpose, clams, Ruditapes philippinarum and R. decussatus, collected from different production areas and found to be infected with Perkinsus sp. in thioglycolate culture medium, were selected for PCR-RFLP tests and sequencing. Perkinsus olseni was detected in all the investigated areas and results also suggested the presence of P. chesapeaki in Leucate, a lagoon on the Mediterranean coast and in...
Tipo: Text Palavras-chave: Clam parasites; Perkinsus chesapeaki; Perkinsus olseni; Ruditapes philippinarum; Ruditapes decussatus; Ribosomal RNA; Actin; ISH.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00094/20542/18205.pdf
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Utilisation d'eaux salées souterraines en baie de Bourgneuf, pour le prégrossissement intensif de mollusques filtreurs en nourricerie ArchiMer
Baud, Jean-pierre.
L'utilisation d'une eau salée souterraine riche en sels nutritifs et de température constante a permis d'étudier l'influence des paramètres physicochimiques essentiels sur la croissance intensive de naissains de Crassostrea gigas et de Ruditapes philippinarum en nourricerie durant les périodes hivernales et estivales. Après la mise au point d'un procédé fiable de culture d'une algue fourrage, la variabilité saisonnière des composés biochimiques de la diatomée Skeletonema costatum produite en grands volumes extérieurs (50 m3) est étudiée. Les conditions d'élevage lors des différentes expériences sont contrôlées (densité de naissain, débit d'eau de mer, concentration de phytoplancton et température). Les différents résultats biologiques obtenus sont analysés...
Tipo: Text Palavras-chave: Ruditapes philippinarum; Crassostrea gigas; Prégrossissement intensif; Nourricerie; Eau salée souterraine; Baie de Bourgneuf; Relations trophiques.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00441/55217/56691.pdf
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Stratégie de prégrossissement intensif de naissains de palourdes (R. philippinarum) et d'huîtres (C. gigas) en baie de Bourgneuf ArchiMer
Baud, Jean-pierre.
Du naissain de palourde japonaise et d'huître creuse a été prégrossi en conditions intensives de nourricerie au moyen d'une eau salée souterraine, utilisée comme ressource thermique et comme source de sels nutritifs pour la production d'algue fourrage. Les paramètres essentiels du milieu ont été suivis et les conditions d'élevage ont été contrôlées (densité de naissain, débit d'eau de mer, concentration de phytoplancton et température). Les résultats biologiques obtenus après traitement des interactions et de la hiérarchie des paramètres étudiés ont fait l'objet d'une analyse économique, pour chaque saison, par rapport à différentes stratégies de prégrossissement des coquillages. Cette étude permet finalement de proposer une méthodologie intensive de...
Tipo: Text Palavras-chave: Baie de Bourgneuf; Ruditapes philippinarum; Crassostrea gigas; Prégrossissement; Nourricerie; Culture intensive.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00048/15892/13314.pdf
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Elevage intensif de la palourde japonaise Ruditapes philippinarum dans les marais atlantiques: faisabilité biologique ArchiMer
Baud, Jean-pierre; Glize, Philippe; Haure, Joel; Garnier, Jacqueline.
Dans un élevage Intensif en claires expérimentales de palourdes japonaises semées à la taille de 28 mm et aux densités de 100, 200 et 300/m2, différentes rations alimentaires de la diatomée Skeletonema costatum. cultivée à partir d'eau salée souterraine, ont été testées de mars à novembre 1988. Pour la densité 100/m2, le taux de croissance n'est pas significativement différent avec des concentrations de pigment dans l'eau injectée comprises entre 31 et 126 µg.l(1) de Chia. Toutefois celui-ci est 1,5 fois supérieur à celui observé dans la claire témoin. Avec 1.8 kg.m-2 la biomasse finale représente 2 fois la charge récoltée dans la claire extensive. Après analyse, la densité de 300/m2 semble être le meilleur compromis pour l'élevage intensif même si la...
Tipo: Text Palavras-chave: Aquaculture intensive; Palourde japonaise; Ruditapes philippinarum; Marais maritime; Zootechnie; Densité; Croissance; Biomasse; Production; Rendement.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00048/15885/13307.pdf
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Intensive culture of the Manila clam (Ruditapes philippinarum) in marine ponds. The impact of rearing densities and feeding levels on growth rates and yields ArchiMer
Baud, Jean-pierre; Haure, Joel; Bodoy, Alain.
Intensive ongrowing (from 28 mm to the commercial size, 35 mm) of the Manila clam Ruditapes philippinarum was studied in experimental raceways with a natural bottom of clay. The factors controlled were rearing density (100, 200, and 300 individuals/m2) and food concentration (34.0 and 68.1 µg.1-1 chlorophyll a). Food was produced from a batch culture of Skeletonema costatum in a large volume (50 m3), thanks to the fertile ground and saline water. Best results regarding final size were obtained in the vicinity of the inflow with a densilY of 100 individuals/m2. However it was a density of 300 individuals/m2 which appeared from the following results to be the optimal choice for intensive rearing: the survival rate was 84 %, the final biomass was 4 kg/m2 and...
Tipo: Text Palavras-chave: Ruditapes philippinarum; Skeletonema costatum; Elevage intensif; Marais maritime; Croissance; Rendement; Ruditapes philippinarum; Skeletonema costatum; Intensive rearing; Marine pond; Growth rate; Yield.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/00038/14950/12276.pdf
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Effets de l'apport de phytoplancton (Skeletonema costatum) sur la croissance et les variations de la composition biochimique de la palourde japonaise Ruditapes philippinarum cultivée en marais ArchiMer
Baud, Jean-pierre; Haure, Joel; Garnier, Jacqueline.
A bulk production of phytoplankton (Skelelonema costatum) has been obtained in concrete tanks from saline groundwater. This was used in various concentrations to feed an intensive cultivation of the Manila clam (Ruditapes philippinarum) in ponds. No difference was observed in growth rates of molluscs, with food concentrations between 31 to 126 µg/l Chl a. However, the growth rates obtained from March to November with a food supplement were 1.5 times higher than those observed in the traditional, extensive cultivation. The biomass doubled and the increase in flesh dry weight was 2.5 times higher. The condition index reached a value of 100 in the intensive cultivation. During growth, the proximate composition analysis revealed that the lipid and carbohydrate...
Tipo: Text Palavras-chave: Ruditapes philippinarum; Skeletonema costatum; Elevage intensif; Claire; Croissance; Composition biochimique; Ruditapes philippinarum; Skeletonema costatum; Intensive rearing; Pond; Growth; Biochemical changes.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00039/14980/12307.pdf
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Optimisation du diagnostic et épidémiologie de la maladie de l'anneau brun sur la côte Atlantique ArchiMer
Beguier, Jeremy.
A l'échelle nationale, la conchyliculture a connu des épizooties catastrophiques ; l'huître portugaise (Crassostrea angulata) a disparu des côtes françaises, suite à des infections impliquant des iridovirus. L'huître plate (Ostrea edulis) a vus sa production fortement réduite suite à l'introduction de deux maladies parasitaires, la bonamiose et la marteiliose. La maladie de l'anneau brun, maladie d'origine bactérienne due à Vibrio tapetis a engendré depuis 1987 en Europe des mortalité allant de 60 à 80% dans les élevages, écloseries et bancs naturels de palourdes japonaises Ruditapes philippinarum et de palourdes européennes R. decussatus, compromettant le développement de cette filière aquacole. Cette étude a pour but d'obtenir une image de la maladie de...
Tipo: Text Palavras-chave: Palourdes; Ruditapes philippinarum; Ruditapes decussatus; Anneau brun; Vibrio tapetis; Biologie moléculaire.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/00034/14480/11780.pdf
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Modélisation de la croissance de Ruditapes philippinarum dans un système expérimental ArchiMer
Bensch, Alexis; Bacher, Cedric; Baud, Jean-pierre; Martin, Jean-louis.
The growth of Ruditapes philippinarum was modeled with an energetic budget equation. Clams were fed with Skeletonema costatum in experimental units during 6 months. The filtration and respiration terms of the budget were depending on the temperature and the dry weight. The spawning effort was represented by an allometric relation derived from the experimental data. The model simulated the individual growth of the dry weight and of the shell converted into energy during the 6 months of the experiment. The results fitted well with the observations until the loss of energy due to the spawning. After that period the model overestimated the growth which was explained by another spawning loss not taken into account in the model and not noticeable with the...
Tipo: Text Palavras-chave: Intensive rearing; Spawning; Sensitivity analysis; Ruditapes philippinarum; Energetic modeling; Elevage intensif; Ponte; Analyse de sensibilité; Ruditapes philippinarum; Modéle énergétique.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-3062.pdf
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Development of culture techniques for new bivalve species ArchiMer
Bodoy, Alain; Mercer, J. P..
Were are two main reasons for the development of cultivation techniques of new bivalve species. First, the risk exists that traditionally reared species may be ereadicated or severely depleted by pathogenic agents. For example the Portuguese 0yster Crassostrea angulata. disapeared from the French coasts in the early 1970's and worldwide production of the flat oyster, Oslrea edulis has been dramatically reduced by the effects of Bonamia. Secondly, in some countries production may he self-Iimiting by the size and availabilily of national and international markets and a wider species range could offer some promising new economic opportunities for aquaculturists. In particular this applies to species where there are identifiable market shortfalls.
Tipo: Text Palavras-chave: Haliotis tuberculata; Ostrea edulis; Placopecten magellanicus; Scallops; Ruditapes philippinarum; Manila clam; Molluscs; Cultivation techniques.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/publication-3033.pdf
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Comparaison de la respiration chez Ruditapes decussatus (L.) et R. philippinarum (Adams & Reeve) en fonction de la température ArchiMer
Bodoy, Alain; Riva, Alain; Maitre-allain, Thierry.
Measurements of oxygen consumption in the two species Ruditapes decussatus and Ruditapes phi/ippinarum were perfonned using hatchery-reared juveniles, grown under controlled conditions. The temperature range varied from 10 to 30°C. The linear relations between the logarithms of the dry weight and of the oxygen consumption were calculated for each species. Rates were compared in terms of standardized clam tissue weight calculated from the regression of respiration on tissue weight. Such a technique allows to make comparisons between the species by avoiding any interference from the variations in individual weight. Within this range of temperature, oxygen consumption of R. philippinarum and of R. decussatus exhibit the same variations, with a maximum at...
Tipo: Text Palavras-chave: Temperature; Oxygen consumption; Ruditapes philippinarum; Ruditapes decussatus; Température; Consommation oxygène; Ruditapes philippinarum; Ruditapes decussatus.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/1986/publication-2963.pdf
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Estimation des stocks de palourdes Ruditapes decussatus, Ruditapes philippinarum sur l'estran oléronais du bassin de Marennes-Oléron. Juin 2009 ArchiMer
Bordeyne, François; Robert, Stephane; Le Moine, Olivier; Leaute, Jean-pierre.
On French coast, Manila clam is a bivalve subject of a large activity recreational and professional hand harvest. Two species are represented in France, European Manila clam Ruditapes decussatus (native) and Japanese Manila clam Ruditapes philippinarum (introduced). Predominance of this last species (more than 90 % of number and biomass), earn them the status of invasive species. Contrary to Arcachon bay, Pont l’Abbé River or Morbihan bay, an estimation campaign of Manila clams stock on Marennes-Oléron bay had never been carried out. The objective of this study: estimate, in number and biomass, the Manila clams stock of two areas. The first affect recreational hand harvest, on Saint-Trojan shore (0.8 km²) whereas the second scopes professional hand...
Tipo: Text Palavras-chave: Palourde; Ruditapes philippinarum; Ruditapes decussatus; Estimation; Stock; Marennes–Oléron.; Manilla clam; Ruditapes philippinarum; Ruditapes decussatus; Estimation; Stock; Marennes–Oleron.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00415/52621/53459.pdf
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Optimisation de l'élevage intensif de la palourde japonaise Ruditapes philippinarum en marais ArchiMer
Breton, Gildas; Baud, Jean-pierre; Haure, Joel.
Après les études de mise au point de la technique intensive réalisées en 1988 et 1989 sur des bassins expérimentaux de 30 m2, une optimisation de cette méthode d'élevage a été faite sur deux claires de 200 m2 avec les normes zootechniques suivantes: densité = 300 palourdes x m-2, concentration d'eau de mer enrichie en Skeletonema costatum produit à partir d'eau salée souterraine = 300 cellules/µl ou 126 µg/l de chla, taux de renouvellement = 100 % du volume par jour. En 8 mois d'élevage, le poids moyen des palourdes est passé de 5,5 g à 19,3 g, soit un rendement de 4,53 kg/m2 pour une production finale de 1,86 tonne, avec une chair de qualité satisfaisante, riche en glycogène et ayant un indice de condition de 94. Aucune trace d'anneau brun n'a été...
Tipo: Text Palavras-chave: Aquaculture intensive; Marais maritime; Ruditapes philippinarum; Skeletonema costatum; Eau salée souterraine; Asperseur.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00048/15880/13302.pdf
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Campagne d'évaluation du stock de palourdes du bassin d'Arcachon - Année 2006 ArchiMer
Caill-milly, Nathalie; Duclercq, Benoit; Morandeau, Gilles.
Between 1980 and 1986, some Arcachon oyster farmers took up breeding the Japanese clam on the foreshore (Robert et Deltreil, 1990). This production was short lived. The steep fall in prices due to economic competition from Spain, Portugal, Italy and Tunisia, predator action such as the trigger fish (Popovsky et al., 1999), added to the significant amount of mortality that occurred in the summer and autumn of 1992 that affected two-thirds of the total stock, led to clam farming being abandoned at the end of the 1980s. In the Arcachon basin, the Japanese clam (Ruditapes philippinarum) population, now natural to the area, has found favourable conditions for development and is expanding rapidly there. This species has been supplanting the local species...
Tipo: Text Palavras-chave: Ruditapes philippinarum; Veneridae; Popluation structure; Biomass; Stock assessment; Arcachon Bassin; Veneridae; Ruditapes Philippinarum; Bivalvia; Mollusca; Structure Population; Biomasse; Abondance; Evaluation Stock.
Ano: 2006 URL: https://archimer.ifremer.fr/doc/2006/rapport-2218.pdf
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Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis ArchiMer
Choquet, Gwenaelle; Soudant, P; Lambert, C; Nicolas, Jean-louis; Paillard, C.
Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity...
Tipo: Text Palavras-chave: Cytotoxicity; Flow cytometry; Vibrio tapetis; Ruditapes philippinarum; Bivalve hemocytes; Pathogenicity.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00000/10761/7590.pdf
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Présence du bivalve Veneridae Ruditapes philippinarum (Adams & Reeve, 1850) sur le littoral sud de la Tunisie OceanDocs
Chouba, L.; Zamouri-Langar, N.; Jarboui, O.; Mrabet, R..
Dans cette étude nous signalons la présence d’une espèce d’origine indo-pacifique, Ruditapes philippinarum, sur les côtes tunisiennes, dans le golfe de Gabès. Les échantillons prélevés ont montré des pourcentages de 89 pour l’espèce indigène Ruditapes decussatus et 11 pour l’espèce exotique Rudiapes philippinarum à Gargour (au sud de Sfax); alors que ces pourcentages sont respectivement de 21,5 et de 78,5 au nord de cette région, à El Aouabed.
Tipo: Journal Contribution Palavras-chave: Ruditapes philippinarum; Geographical distribution; New records; Marine molluscs; Geographical distribution; Http://aims.fao.org/aos/agrovoc/c_5083.
Ano: 2006 URL: http://hdl.handle.net/1834/4274
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Development of a generic decision-support tool for identifying potentially invasive aquatic taxa: AS-ISK ArchiMer
Copp, Gordon H.; Vilizzi, Lorenzo; Tidbury, Hannah; Stebbing, Paul D.; Trakan, Ali Serham; Miossec, Laurence; Goulletquer, Philippe.
Electronic decision-support tools are now an essential component of government strate gies to battle non-native species, with taxon-specific, paper-based risk analysis schemes often being replaced by taxon-generic tools. This study reports on the development of a decision-support tool for aquatic species, the Aquatic Species Invasiveness Screening Kit (AS-ISK), which replaces five taxon-specific toolkits for amphibians, freshwater and marine fish and invertebrates (FISK, MFISK, FI-ISK, MI-ISK, Amph-ISK). Adapted from Pheloung et al.’s. Weed Risk Assessment (WRA), the “-ISK” toolkits were also “self-automated workbooks” in Excel® VisualBasic® architecture of the ’WRA. In creating AS-ISK, we incorporated the conceptual approach (questions and guidance) of...
Tipo: Text Palavras-chave: Risk analusis; Marine; Brackish; FISK; Ruditapes philippinarum.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00357/46770/46638.pdf
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Brown muscle disease (BMD), an emergent pathology affecting Manila clam Ruditapes philippinarum in Arcachon Bay (SW France) ArchiMer
Dang, C; De Montaudouin, X; Gonzalez, P; Mesmer Duldons, N; Caill-milly, Nathalie.
We describe an emerging pathology, brown muscle disease (BMD), which specifically affects the Manila clam Ruditapes philippinarum in Arcachon Bay (France). BMD induces a transformation of the posterior adductor muscle, which becomes infused by conchiolin and calcified, reducing the ability of clams to bury. The disease affects both types of muscular tissue, with striated muscle becoming affected to a higher degree than smooth muscle. Two indices were created to quantify the symptoms: the Muscle Print Index, used for empty and live shells, and the Final Disease Index, utilized for live clams only. Histological sections were made and observed under light microscopy to examine the muscular damage and to investigate a causal agent. Sections revealed an...
Tipo: Text Palavras-chave: Light microscopy; Pathology; Adductor muscle; Ruditapes philippinarum; Clam; Brown muscle disease.
Ano: 2008 URL: https://archimer.ifremer.fr/doc/2008/publication-4556.pdf
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Virus-like particles associated with brown muscle disease in Manila clam, Ruditapes philippinarum, in Arcachon Bay (France) ArchiMer
Dang, C.; Gonzalez, P.; Mesmer-dudons, N.; Bonami, J-r; Caill-milly, Nathalie; De Montaudouin, X..
Recently, Manila clam, Ruditapes philippinarum, populations have suffered mortalities in Arcachon Bay (SW France). Mortality was associated with extensive lesions of the posterior adductor muscle, which become progressively brown and calcified. Ultrastructural observations by transmission electron microscopy revealed tissue degradation with necrotized muscle fibres and granulocytomas. Unenveloped virus-like particles (VLPs) were detected in muscle, granulocytic, epithelial and rectal cells. VLPs were abundant in the extracellular space, in the cytoplasm (free or enclosed in vesicles) and in the nucleoplasm of granulocytes. Nuclei and mitochondria of granulocytes displayed changes which suggested reactive oxygen species production and apoptosis induction....
Tipo: Text Palavras-chave: Adductor muscle; Apoptosis; Brown muscle disease; Necrosis; Ruditapes philippinarum; Transmission electron microscopy.
Ano: 2009 URL: https://archimer.ifremer.fr/doc/00000/11130/7991.pdf
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Determination of diet in Manila clams by spatial analysis of stable isotopes ArchiMer
Dang, C.; Sauriau, Pierre-guy; Savoye, N.; Caill-milly, Nathalie; Martinez, P.; Millaret, C.; Haure, Joel; De Montaudouin, X..
Spatial changes in the dietary regime of Ruditapes philippinarum from intertidal sediments of Arcachon Bay were depicted by analyzing stable isotope ratios in both its adductor muscles and potential trophic sources. Manila clams were collected from 50 sites in May to June 2006. Dietary reconstruction was based on the IsoSource mixing model, considering trophic enrichments of 3.5%. for carbon and 3.0%. for nitrogen, which were determined experimentally. delta C-13 and delta N-15 values, respectively, averaged -28.2 and 5.2 parts per thousand for riverine particulate organic matter (POM), -20.6 and 4.7 parts per thousand for inner bay phytoplankton, -21.0 and 5.6%. for outer bay phytoplankton, -16.8 and 4.8 parts per thousand. for microphytobenthos, -18.4...
Tipo: Text Palavras-chave: SW France; Arcachon Bay; Spatial variability; Mixing model; Trophic sources; C and N stable isotopes; Muscle; Ruditapes philippinarum.
Ano: 2009 URL: https://archimer.ifremer.fr/doc/2009/publication-6886.pdf
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